Mouse monoclonal [F8-11-13] antibody to CD45RA (Amethyst Orange).
Specificity
This antibody recognises the human CD45RA cell surface antigen. CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc. Mouse anti Human CD45RA antibody, clone F8-11-13 reacts with the high molecular weight form of the leucocyte common antigen (LCA) expressed by B lymphocytes and a subset of T lymphocytes. Mouse anti Human CD45RA antibody, clone F8-11-13 is routinely tested in flow cytometry on human peripheral blood lymphocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1:50 - 1:100, IHC-P: 1:200, Use 10µl of the suggested working dilution to label 106 cells in 100µl. This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Reactivity
Human, Rhesus Monkey
Immunogen
Human T lymphocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
F8-11-13
Isotype
IgG1
Conjugate
Amethyst Orange
Excitation: 390nm, Emission: 535nm
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Human CD45RA antibody, clone F8-11-13 recognizes the human CD45RA cell surface antigen. CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc.Mouse anti Human CD45RA antibody, clone F8-11-13 reacts with the high molecular weight form of the leucocyte common antigen (LCA) expressed by B lymphocytes and a subset of T lymphocytes.Mouse anti Human CD45RA antibody, clone F8-11-13 is routinely tested in flow cytometry on human peripheral blood lymphocytes.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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