This antibody recognises an epitope contained in the portion of porcine CD45 encoded by exon A, CD45RA. This antibody recognises both the 210 kDa RA CD45 isoform and the 226 kDa RAC isoform. Clone MIL13 does not recognize the CD45RC or CD45RO isoforms.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Porcine
Immunogen
Cells isolated from porcine mesenteric lymph node.
Host
Mouse
Clonality
Monoclonal
Clone ID
MIL13
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Figure A. Anti-CD8 Beta Antibody [PPT23] (FITC) (A281586) and Mouse IgG1 (PE) - Isotype Control (A282643). Figure B. Anti-CD8 Beta Antibody [PPT23] (FITC) (A281586) and Anti-CD45RA Antibody [MIL13] (PE) (A281200). All experiments performed on red cell lysed porcine blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.
Figure A. Anti-CD52 Antibody [11/305/44] (FITC) (A278957) and Mouse IgG1 (PE) - Isotype Control (A282643). Figure B. Anti-CD52 Antibody [11/305/44] (FITC) (A278957) and Anti-CD45RA Antibody [MIL13] (PE) (A281200). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Figure A. Anti-CD45RA Antibody [MIL13] (PE) (A281200) and Mouse IgG1 (FITC) - Isotype Control (A282453). Figure B. Anti-CD45RA Antibody [MIL13] (PE) (A281200) and Anti-CD52 Antibody [11/305/44] (FITC) (A278957). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
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