Anti-CD45RA Antibody [OX-33] (FITC) (A281437)

Anti-CD45RA Antibody [OX-33] (FITC)

Mouse monoclonal [OX-33] antibody to CD45RA (FITC).

This antibody recognises a high molecular weight band of the leucocyte common antigen. MRC OX-33 only labels B-cells among thoracic duct lymphocytes with little labeling in bone marrow and none on thymocytes.

Flow Cytometry: Neat - 1:10, This antibody does not always give a clear separation between positives and negatives in some cell preparations. If users find this to be the case we recommend the use of R. Phycoerythrin conjugated reagent MCA340PE which generally exhibits a peak of higher mean fluorescence. Use 10µl of the suggested working dilutions to label 10⁶ cells in 100µl.

Rat

Purified rat spleen L-CA.

Mouse

Monoclonal

IgG1

Protein G affinity chromatography of tissue culture supernatant.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse anti Rat CD45RA antibody, clone OX-33 is directed against a high molecular weight band of the leucocyte common antigen. MRC OX-33 only labels B-cells among thoracic duct lymphocytes with little labeling in bone marrow and none on thymocytes (Barclay et al. 1987).

This product is for research use only. It is not intended for diagnostic or therapeutic use.