This antibody recognises the RB isoform of the bovine leucocyte common antigen expressed on subpopulations of CD4+ and CD8+ T cells. Expression levels of CD45RB are used as a marker for differentiating naïve and memory T lymphocytes. The antigen recognized by clone CC76 is also expressed on B cells but is absent from both macrophages and granulocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1:100 - 1:200, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Bovine
Immunogen
Bovine thymocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
CC76
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Bovine CD45RB, clone CC76, recognizes the RB isoform of the bovine leucocyte common antigen expressed on subpopulations of CD4+ and CD8+ T cells. Expression levels of CD45RB are used as a marker for differentiating naïve and memory T lymphocytes (Howard et al. 1991). The antigen recognized by clone CC76 is also expressed on B cells but is absent from both macrophages and granulocytes.