Anti-CD45RO Antibody [UCHL-1] (CF®405S) (A352086)

Anti-CD45RO Antibody [UCHL-1] (CF®405S)

Mouse monoclonal [UCHL-1] antibody to CD45RO (CF®405S).

Functional studies, Flow Cytometry (Cell Surface), IF, IHC, IHC-P, IP, WB

CCRF-CEM, Jurkat, and MOLT-4 cells or tonsil paracortex and reactive lymph node.

Flow Cytometry: 0.5-1 µg/million cells in 100 µL, IF: 0.5-1 µg/mL, IHC-P: 0.25-0.5 µg/mL. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.

Human, Marmoset, Chimpanzee

Clone UCHL-1: Cultured human T cells from the IL-2-dependent CA1 T-cell line.
Clone T200/797: Recombinant human CD45RO protein.

Mouse

Monoclonal

IgG2a

kappa

0.1 mg/mL

180-185 kDa

Liquid

Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.

Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.

This product is for research use only. It is not intended for diagnostic or therapeutic use.