This antibody recognises the low molecular weight isoform (180 kDa) of the leucocyte common antigen (LCA). The antigen is expressed by a functional subset of T cells with memory phenotype.In peripheral blood Mouse anti Human CD45RO, clone UCHL1 stains 40-80% of lymphocytes and all monocytes and granulocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Human, Chimpanzee, Marmoset
Immunogen
Human IL-2 dependent T cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
UCHL1
Isotype
IgG2a
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Human CD45RO monoclonal antibody, clone UCHL1 recognizes the low molecular weight isoform (180 kDa) of the leucocyte common antigen (LCA). The antigen is expressed by a functional subset of T cells with memory phenotype.In peripheral blood Mouse anti Human CD45RO, clone UCHL1 stains 40-80% of lymphocytes and all monocytes and granulocytes.
Figure A. A488 conjugated mouse anti human CD45RA and Mouse IgG2a (PE) - Isotype Control (A282644). Figure B. A488 conjugated mouse anti human CD45RA and Anti-CD45RO Antibody [UCHL1] (PE) (A281868). All experiments performed on red cell lysed human blood gated on lymphocytes. Data acquired on the ZE5 cell analyzer.
Figure A. Anti-CD45RO Antibody [UCHL1] (PE) (A281868). Figure B. Anti-CD45RO Antibody [UCHL1] (PE) (A281868) and SBV515 conjugated mouse anti Human CD45RA. All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Figure A. Anti-CD45RO Antibody [UCHL1] (PE) (A281868). Figure B. Anti-CD45RO Antibody [UCHL1] (PE) (A281868) and StarBright Violet 790 conjugated mouse anti Human CD45RA. All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Figure A. Anti-CD45RO Antibody [UCHL1] (PE) (A281868). Figure B. Anti-CD45RO Antibody [UCHL1] (PE) (A281868)) and StarBright Blue 675 conjugated mouse anti Human CD45RA. All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
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