Mouse monoclonal [MEM-102] antibody to CD48 (FITC).
Specificity
This antibody reacts with CD48, a 40-47 kDa GPI-anchored extracellular membrane protein of the immunoglobulin supergene family, widely expressed on hematopoietic cells. It is negative on granulocytes, platelets, and erythrocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity
Human, Non-Human Primates
Immunogen
Raji human Burkitt's lymphoma cell line.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-102
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
This antibody is conjugated with Fluorescein Isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
40-47 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
B-lymphocyte activation marker BLAST-1, BCM1, BCM1 surface antigen, BLAST1, CD48 antigen, Leukocyte antigen MEM-102, Signaling lymphocytic activation molecule 2, SLAM family member 2, SLAMF2, TCT.1
Separation of human CD48 positive lymphocytes (red-filled) from human CD48 negative neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using Anti-CD48 Antibody [MEM-102] (FITC) (A86026)(20 µl reagent / 100 µl of peripheral whole blood).
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Proteins predicted to interact with CD48
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.