This antibody recognises the intact complex formed between the CD51 and CD61 molecules (alpha V and beta 3 integrins. It reacts with osteoclasts, placenta, melanoma cell lines and weakly with platelets.
Applications
IF, IP, Flow Cytometry, IHC-Fr
Dilutions
Flow Cytometry: 1:40 - 1:80, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Human, Chicken
Cross Reactivity
This antibody does not cross react with Bovine, Pig, or Rabbit
Immunogen
Osteoclasts from osteoclastomas.
Host
Mouse
Clonality
Monoclonal
Clone ID
23C6
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
The complex between the CD51 and CD61 molecules (alpha V and beta 3 integrins) binds vitronectin at the RGD sequence and can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen.
