Anti-CD58 Antibody [BRIC5] (A279864)

Anti-CD58 Antibody [BRIC5]

Mouse monoclonal [BRIC5] antibody to CD58.

This antibody recognises human Lymphocyte function-associated antigen 3, also known as CD58 or LFA-3. CD58 is a 250 amino acid single pass type I transmembrane glycoprotein, a member of the immunoglobulin superfamily, with a predicted molecular mass of 28.1 kDa and an apparent molecular mass of ~55-70 kDa. CD58 occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA and is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes. CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells. There are approximately 5,000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria. Mouse anti Human CD58 antibody, clone BRIC5 was produced in response to erythrocytes. The functional affinity of BRIC5 binding to erythrocytes is 4 x 108 M-1. It reacts by immunoblotting to non-reduced erythrocyte membranes. BRIC5 is an indirect haemagglutinin. The antigen on erythrocytes is pronase sensitive. Mouse anti Human CD58 antibody, clone BRIC5 inhibits T cell rosetting.

WB, Flow Cytometry

Flow Cytometry: 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Human

Human erythrocytes.

Mouse

Monoclonal

BRIC5

IgG2a

1 mg/ml

Liquid

Supplied in TRIS buffered glycine with <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CD58 antibody, clone BRIC5 recognizes human Lymphocyte function-associated antigen 3, also known as CD58 or LFA-3. CD58 is a 250 amino acid single pass type I transmembrane glycoprotein, a member of the immunoglobulin superfamily, with a predicted molecular mass of 28.1 kDa and an apparent molecular mass of ~55-70 kDa. CD58 occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA and is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes (Makgoba et al. 1989).CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells. There are approximately 5,000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria.Mouse anti Human CD58 antibody, clone BRIC5 was produced in response to erythrocytes. The functional affinity of BRIC5 binding to erythrocytes is 4 x 108 M-1. It reacts by immunoblotting to non-reduced erythrocyte membranes. BRIC5 is an indirect haemagglutinin. The antigen on erythrocytes is pronase sensitive. Mouse anti Human CD58 antibody, clone BRIC5 inhibits T cell rosetting.

Ag3, LFA3, Lymphocyte function-associated antigen 3, Surface glycoprotein LFA-3