This antibody reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues. The MEM-43/5 does not compete with most other CD59 antibodies.
Western blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL human peripheral blood T cell leukemia cell line using Anti-CD59 Antibody [MEM-43/5] (A85695). Lane 1: original cell lysateLane 2: material immunoprecipitated with Anti-CD59 Antibody [MEM-43] (A85989).
Separation of human neutrophil granulocytes (red-filled) from human CD59 negative blood debris (black-dashed) in flow cytometry surface staining of human peripheral blood, labeling CD59 with Anti-CD59 Antibody [MEM-43/5] (A85695), (concentration in sample 0.6 µg/ml, GAM APC).