Mouse monoclonal [MEM-259] antibody to CD63 (PerCP-Cyanine 5.5).
Specificity
This antibody reacts with an extracellular / luminal epitope of CD63, a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes / macrophages, and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
HPB-ALL human T cell line.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-259
Isotype
IgG1
Conjugate
PerCP-Cyanine 5.5
Excitation: 482nm, Emission: 695nm
Purification
This antibody is conjugated with tandem dye PerCP-Cyanine 5.5 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
40-60 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
CD63 antigen, Granulophysin, LAMP-3, Limp1, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1, Melanoma-associated antigen ME491, MLA1, OMA81H, TSPAN30
Flow cytometric analysis of IgE activated peripheral blood stained with Anti-CD63 Antibody [MEM-259] (PerCP-Cyanine 5.5).
Publishing research using Anti-CD63 Antibody [MEM-259] (PerCP-Cyanine 5.5) (A121960)? Please let us know so that we can list the citation on this page.
Proteins predicted to interact with CD63
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.