This antibody recognizes CD73, a 70 kDa GPI-anchored 5´-nucleotidase expressed predominantly on the surface of T and B cell subsets, follicular dendritic cells, and endothelial cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human, Non-Human Primates
Immunogen
Unfortunately, this information is considered to be commercially sensitive.
Host
Mouse
Clonality
Monoclonal
Clone ID
AD2
Isotype
IgG1
Light Chains
kappa
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
70 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometric analysis of human peripheral whole blood stained using Anti-CD73 Antibody [AD2] (APC) (10µl reagent per 100µl of peripheral whole blood).
Flow cytometric analysis of human lymphocytes using Anti-CD73 Antibody [AD2] (APC) (10µl reagent per 100µl of peripheral whole blood) and Anti-CD19 Antibody [LT19] (PE) (20µl reagent per 100µl of peripheral whole blood).
Separation of human CD73 positive CD19 positive B cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis of human peripheral whole blood using Anti-CD73 Antibody [AD2] (APC) (10µl reagent per 100µl of peripheral whole blood).
Publishing research using Anti-CD73 Antibody [AD2] (APC) (A121913)? Please let us know so that we can list the citation on this page.
Proteins predicted to interact with CD73
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.