This antibody recognizes CD77, a neutral glycosphingolipid expressed mainly on the surface of B cell populations, such as germinal center B cells and Burkitt´s lymphoma cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Daudi cell line (Burkitt's lymphoma).
Host
Rat
Clonality
Monoclonal
Clone ID
38.13
Isotype
IgM
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 8, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Separation of RAJI cells stained with Anti-CD77 Antibody [38.13] (PE) (10 µl reagent per million cells in 100 µl of cell suspension, red-filled) from RAJI cells stained with Mouse IgG1 [MOPC-21] (APC) - Isotype Control (concentration in sample is 5 µg/ml, same as Anti-CD77 Antibody [38.13] (PE) concentration, black-dashed) in flow cytometry analysis (surface staining) of RAJI cell suspension.
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