Anti-CD8 alpha Antibody [KT15] (Amethyst Orange) (A280824)

Anti-CD8 alpha Antibody [KT15] (Amethyst Orange)

Rat monoclonal [KT15] antibody to CD8 alpha (Amethyst Orange).

This antibody recognises the alpha chain of mouse CD8. CD8 is a heterodimeric protein composed of disulphide-linked CD8a and CD8ß chains that is expressed primarily on cytotoxic T-cells. CD8 functions in the interaction with MHC Class I-bearing targets and plays a role in T-cell-mediated killing (Nakauchi , H. et al., 1985 & Nakauchi, H. et al., 1987).Clone KT15 is reported to block T-cell-mediated cytotoxicity in in vitro assays (Zeis, M. et al., 2002).

Flow Cytometry: 1:50 for 0.25 mg, 25 µg, 1:100 for 0.1 mg - 1:100 for 0.25 mg, 25 µg, 1:200 for 0.1 mg, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Mouse

T cell clone, C6.

Rat

Monoclonal

KT15

Protein G affinity chromatography of tissue culture supernatant.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Rat anti Mouse CD8a, clone KT15, recognizes the alpha chain of mouse CD8. CD8 is a heterodimeric protein composed of disulphide-linked CD8a and CD8ß chains that is expressed primarily on cytotoxic T-cells. CD8 functions in the interaction with MHC Class I-bearing targets and plays a role in T-cell-mediated killing (Nakauchi , H. et al., 1985 & Nakauchi, H. et al., 1987).Clone KT15 is reported to block T-cell-mediated cytotoxicity in in vitro assays (Zeis, M. et al., 2002).

CD8a, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

This product is for research use only. It is not intended for diagnostic or therapeutic use.