Mouse monoclonal [RIV11] antibody to CD8 (Biotin).
Applications
Flow Cytometry (Cell Surface), IF
Positive Control
HuT78 cells and human peripheral blood lymphocytes (hPBL) or tonsil tissue.
Dilutions
Flow Cytometry: 0.5-1 µg/million cells in 100 µL, IF: 0.5-1 µg/mL. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.
Reactivity
Human
Immunogen
Human peripheral lymphocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
RIV11
Isotype
IgG1
Light Chains
kappa
Conjugate
Biotin
Concentration
0.1 mg/mL
Predicted MW
32 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD8 Antibody [RIV11] (CF®647) (red). Fluorescence was detected in the PI channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.
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