Mouse monoclonal [341] antibody to CD8 beta (FITC).
Specificity
This antibody recognises the beta chain of the rat CD8 cell surface antigen, expressed on thymocytes and peripheral cytotoxic/suppressor cells. CD8b is not expressed by NK cells or by intestinal intraepithelial T cells. Mouse anti Rat CD8 beta antibody, clone 341 is reported to block proliferative and cytotoxic responses of CD8 +ve effector cells in vitro.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1:2 - 1:10, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Rat
Immunogen
Rat/Mouse T cell hybrids expressing CD8.
Host
Mouse
Clonality
Monoclonal
Clone ID
341
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Rat CD8 beta antibody, clone 341 recognizes the beta chain of the rat CD8 cell surface antigen, expressed on thymocytes and peripheral cytotoxic/suppressor cells. CD8b is not expressed by NK cells or by intestinal intraepithelial T cells. Mouse anti Rat CD8 beta antibody, clone 341 is reported to block proliferative and cytotoxic responses of CD8 +ve effector cells in vitro.
