This antibody recognises the bovine CD80 cell surface antigen, expressed by dendritic cells, activated macrophages and activated B cells. CD80 plays a key role in co-stimulation of T cells during the primary immune response.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:50, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl
Reactivity
Bovine, Sheep
Host
Mouse
Clonality
Monoclonal
Clone ID
IL-A159
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Bovine CD80 antibody, clone IL-A159 recognizes the bovine CD80 cell surface antigen, expressed by dendritic cells, activated macrophages and activated B cells. CD80 plays a key role in co-stimulation of T cells during the primary immune response.
Figure A. Anti-CD14 Antibody [CC-G33] (FITC) (A280069) and Mouse IgG1 (PE) - Isotype Control (A282643). Figure B. Anti-CD14 Antibody [CC-G33] (FITC) (A280069) and Anti-CD80 Antibody [IL-A159] (PE) (A280719). All experiments performed on red cell lysed bovine blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.
Figure A. Anti-CD80 Antibody [IL-A159] (PE) (A280719) and Mouse IgG1 (FITC) - Isotype Control (A282453). Figure B. Anti-CD80 Antibody [IL-A159] (PE) (A280719) and Anti-CD14 Antibody [CC-G33] (FITC) (A280069). All experiments performed on red cell lysed bovine blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.
Figure A. Anti-CD62L Antibody [CC32] (FITC) (A280038) and Mouse IgG1 (PE) - Isotype Control (A282643). Figure B. Anti-CD62L Antibody [CC32] (FITC) (A280038) and Anti-CD80 Antibody [IL-A159] (PE) (A280719). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Figure A. Anti-CD80 Antibody [IL-A159] (PE) (A280719) and Mouse IgG1 (FITC) - Isotype Control (A282453). Figure B. Anti-CD80 Antibody [IL-A159] (PE) (A280719) and Anti-CD62L Antibody [CC32] (FITC) (A280038). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Figure A. Anti-CD14 Antibody [CC-G33] (FITC) (A280069) and Mouse IgG1 (PE) - Isotype Control (A282643). Figure B. Anti-CD14 Antibody [CC-G33] (FITC) (A280069) and Anti-CD80 Antibody [IL-A159] (PE) (A280719). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.