Anti-CD86 Antibody [PO3] (PE) (A281582)

Anti-CD86 Antibody [PO3] (PE)

Rat monoclonal [PO3] antibody to CD86 (PE).

This antibody recognises mouse CD86 (B7-2), a ~80 kDa cell surface glycoprotein, a member of the CD28/B7 family. In mouse CD86 is expressed at high levels on peripheral blood monocytes and dendritic cells, and at low levels on resting B and T-lymphocytes. Expression of CD86, on these cell populations, can be increased upon activation. CD86 has been identified, along with CD80 (B7-1), as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA4). CD28 and CTLA4 are two receptors that have opposing functions in T-cell stimulation. Interaction of CD86 with CD28 promotes a number of T-cell activities, whereas the binding of CD86 to CTLA4 inhibits T-cell responses. Rat anti mouse CD86 antibody, clone PO3 has been reported to block some co-stimulatory functions of CD86.

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Mouse

Mouse B-cell line, BCL1.

Rat

Monoclonal

PO3

IgG2b

Protein G affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Lyophilized

Pack Size of 100 Tests: Reconstitute with 1 ml of distilled water. Pack Size of 25 Tests: Reconstitute with 0.25 ml of distilled water.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Rat anti mouse CD86 antibody, clone PO3 recognizes mouse CD86 (B7-2), a ~80 kDa cell surface glycoprotein, a member of the CD28/B7 family. In mouse CD86 is expressed at high levels on peripheral blood monocytes and dendritic cells, and at low levels on resting B and T-lymphocytes. Expression of CD86, on these cell populations, can be increased upon activation. CD86 has been identified, along with CD80 (B7-1), as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA4). CD28 and CTLA4 are two receptors that have opposing functions in T-cell stimulation. Interaction of CD86 with CD28 promotes a number of T-cell activities, whereas the binding of CD86 to CTLA4 inhibits T-cell responses. Rat anti mouse CD86 antibody, clone PO3 has been reported to block some co-stimulatory functions of CD86 (Nakajima et al. 1997).

Activation B7-2 antigen, B70, BU63, CD28LG2, CTLA-4 counter-receptor B7.2, FUN-1, T-lymphocyte activation antigen CD86

This product is for research use only. It is not intended for diagnostic or therapeutic use.