Mouse monoclonal [5E10] antibody to CD90/Thy1 (PE).
Specificity
This antibody recognizes CD90, a GPI-anchored cell surface glycoprotein expressed predominantly on thymocytes, hematopoietic stem cells, and neurons.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Porcine, Horse, Human, Non-Human Primates
Cross Reactivity
This antibody does not cross react with Dog.
Immunogen
HEL erythroleukemia cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
5E10
Isotype
IgG1
Light Chains
kappa
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of Jurkat cells (red-filled) from SP2 cells (black-dashed) in flow cytometry analysis (surface staining) stained with Anti-CD90/Thy1 Antibody [5E10] (PE) (A86412), (10 µl reagent per milion cells in 100 µl of cell suspension).
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![Flow Cytometry - Anti-CD90/Thy1 Antibody [5E10] (PE) (A86412)](https://cdn.antibodies.com/image/catalog/86/A86412_1.jpg?profile=product_top)
![Flow Cytometry - Anti-CD90/Thy1 Antibody [5E10] (PE) (A86412)](https://cdn.antibodies.com/image/catalog/86/A86412_1.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-CD90/Thy1 Antibody [5E10] (PE) (A86412)](https://cdn.antibodies.com/image/catalog/86/A86412_1.jpg?profile=product_image)