Rabbit polyclonal antibody to cdc25C (Phospho-Ser216)
Specificity
The antibody detects endogenous level of cdc25C only when phosphorylated at serine 216.
Applications
WB, IHC, IF
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 216 (S-P-S(p)-M-P) derived from Human cdc25C.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25C (Phospho-Ser216) Antibody #11118 (left) or the same antibody preincubated with blocking peptide (right).
![Western Blot - Anti-Cdc25C Antibody [ARC0622] (A307870) - Antibodies.com](https://cdn.antibodies.com/image/catalog/307/A307870_1.jpg?profile=product_alternative)
