Anti-Cdc27 Antibody (A11494)

Name: Anti-Cdc27 Antibody
Brand: Antibodies.com
SKU: A11494
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to Cdc27 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Cdc27 Antibody

Description

Rabbit polyclonal antibody to Cdc27.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 551-830 of human CDC27 (NP_001107563.1).

Sequence

HLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

110 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

ANAPC3, Anaphase-promoting complex subunit 3, APC3, CDC27 homolog, CDC27Hs, Cell division cycle protein 27 homolog, D0S1430E, D17S978E, H-NUC

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Cdc27 Antibody (A11494)

Western blot analysis of extracts of HeLa cells, using Anti-Cdc27 Antibody (A11494) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021).

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Immunohistochemistry - Anti-Cdc27 Antibody (A11494)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Cdc27 Antibody (A11494) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Cdc27 Antibody (A11494)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Cdc27 Antibody (A11494) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Cdc27 Antibody (A11494)

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-Cdc27 Antibody (A11494) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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