This antibody recognizes a conformationally dependent epitope of native CD66c, a GPI-anchored extracellular glycoprotein expressed on granulocytes and epithelial cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Extracts from human breast carcinoma cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
B6.2
Isotype
IgG1
Light Chains
kappa
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained with Anti-CD66c Antibody [B6.2] (PE) (10 µl reagent / 100 µl of peripheral whole blood).
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