Anti-Chk1 Antibody [2G1D5] (A279229)

Anti-Chk1 Antibody [2G1D5]

Mouse monoclonal [2G1D5] antibody to Chk1.

This antibody recognises Chk1 also known as cell cycle checkpoint kinase homologue 1, CHK1 or checkpoint kinase-1.Chk1, like the other checkpoint kinase Chk2, is a serine/threonine protein kinase that plays a crucial role in cell cycle control. Chk1 becomes phosphorylated and consequently activated in response to DNA damage by the ATR kinase. Upon this activation Chk1 phosphorylates a variety of proteins including p53 and Cdc25, which triggers cell cycle arrest, DNA repair and apoptosis. Chk1 plays a critical role in preventing aberrant cells from progressing through the cell cycle. Based on this, it is not surprising that Chk1 is of interest in the field of cancer research and as a therapeutic target.

WB: 1 µg/ml

Human, Mouse, Rat

Recombinant human Chk1.

Mouse

Monoclonal

2G1D5

IgG1

Protein G affinity chromatography of ascites.

Approximately 55 kDa by Western Blot

Liquid

Supplied in Phosphate Buffered Saline with 50% Glycerol and <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Chk1 antibody, clone 2G1D5 recognizes Chk1 also known as cell cycle checkpoint kinase homologue 1, CHK1 or checkpoint kinase-1.Chk1, like the other checkpoint kinase Chk2, is a serine/threonine protein kinase that plays a crucial role in cell cycle control. Chk1 becomes phosphorylated and consequently activated in response to DNA damage by the ATR kinase. Upon this activation Chk1 phosphorylates a variety of proteins including p53 and Cdc25, which triggers cell cycle arrest, DNA repair and apoptosis (Bartek and Lukas, 2003).Chk1 plays a critical role in preventing aberrant cells from progressing through the cell cycle. Based on this, it is not surprising that Chk1 is of interest in the field of cancer research and as a therapeutic target (Thompson and Eastman, 2013).

Cell cycle checkpoint kinase, Checkpoint kinase-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1