Rabbit polyclonal antibody to Chk2 (Phospho-Thr383)
Specificity
The antibody detects endogenous levels of Chk2 only when phosphorylated at threonine 383.
Applications
WB, ICC/IF
Dilutions
Western blotting: 1:500~1:1000 Immunofluorescence: 1:100~1:200
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
Peptide sequence around phosphorylation site of threonine383 (M-R-T(p)-L-C) derived from Human Chk1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from COS7 cells treated with UV using Chk2 (Phospho-Thr383) Antibody #11683.The lane on the right is treated with the antigen-specific peptide.
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![SDS-PAGE - Anti-CHEK2 Antibody [PCRP-CHEK2-1A4] (A277540) - Antibodies.com](https://cdn.antibodies.com/image/catalog/277/A277540_1.jpg?profile=product_alternative)
