Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
C20orf178, Charged multivesicular body protein 4b, Chromatin-modifying protein 4b, hSnf7-2, hVps32-2, SHAX1, SNF7 homolog associated with Alix 1, SNF7-2, Vacuolar protein sorting-associated protein 32-2, Vps32-2
Western blot analysis of extracts of various cell lines, using Anti-CHMP4B Antibody (A15682) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using Anti-CHMP4B Antibody (A15682) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunofluorescence analysis of NIH/3T3 cells using Anti-CHMP4B Antibody (A15682) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
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