Western blot analysis of extracts of various cell lines, using Anti-CIKS/ACT1 Antibody (A12017) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Western blot analysis of extracts of Rat lung, using Anti-CIKS/ACT1 Antibody (A12017) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunofluorescence analysis of NIH/3T3 cells using Anti-CIKS/ACT1 Antibody (A12017) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of PC-12 cells using Anti-CIKS/ACT1 Antibody (A12017) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U2OS cells using Anti-CIKS/ACT1 Antibody (A12017) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
