Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Cytokine-induced protein of 29 kDa, HCC1, Nuclear protein Hcc-1, Proliferation-associated cytokine-inducible protein CIP29, SAP domain-containing ribonucleoprotein, SARNP
Western blot analysis of extracts of various cell lines, using Anti-CIP29 Antibody (A89103) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-CIP29 Antibody (A89103) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
