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Anti-CISH/CIS Antibody (A87630)

Western Blot - Anti-CISH/CIS Antibody (A87630) - Antibodies.com Immunofluorescence - Anti-CISH/CIS Antibody (A87630) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to CISH/CIS for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-CISH/CIS Antibody
Description
Rabbit polyclonal antibody to CISH/CIS.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 17-130 of human CISH (NP_659508.1).
Sequence
TGQRPLWAPSLELPKPVMQPLPAGAFLEEVAEGTPAQTESEPKVLDPEEDLLCIAKTFSYLRESGWYWGSITASEARQHLQKMPEGTFLVRDSTHPSYLFTLSVKTTRGPTNVR
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
37 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-CISH/CIS Antibody (A87630) - Antibodies.comEnlarge Image

Western Blot - Anti-CISH/CIS Antibody (A87630)

Western blot analysis of extracts of 293T cells, using Anti-CISH/CIS Antibody (A87630) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 15s.
Immunofluorescence - Anti-CISH/CIS Antibody (A87630) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-CISH/CIS Antibody (A87630)

Immunofluorescence analysis of C6 cells using Anti-CISH/CIS Antibody (A87630) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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