Rabbit polyclonal antibody to CK2a (phospho-Thr360 / Ser362)
Specificity
The antibody detects endogenous level of CK2 only when phosphorylated at thronine 360.
Applications
WB
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of threonine360 / serine 362 (V-P-T(p)-P-S(p)-P-L) derived from Human CK2a.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot of CK2a (Phospho- Thr360/Ser362) antibody (#11572) and CK2a antibody (#21572) in vitro kinase assay. Both purified ERK2 and CK2 were used. CK2a (Phospho-Thr360/Ser362) antibody could recognize ERK2 phosphorylated wild type CK2a and CK2a when Ser362 was mutated to alanine .
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