Western blot analysis of extracts of various cell lines, using Anti-CKAP4 Antibody (A15890) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Confocal immunofluorescence analysis of U-2 OS cells using Anti-CKAP4 Antibody (A15890) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
