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Anti-Cleaved PARP1 Antibody [ARC0091] (A308342)

Western Blot - Anti-Cleaved PARP1 Antibody [ARC0091] (A308342) - Antibodies.com Western Blot - Anti-Cleaved PARP1 Antibody [ARC0091] (A308342) - Antibodies.com
$515
Product Datasheet

Rabbit monoclonal [ARC0091] antibody to Cleaved PARP1 for WB and IP.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Cleaved PARP1 Antibody [ARC0091]
Description
Rabbit monoclonal [ARC0091] antibody to Cleaved PARP1.
Applications
WB, IP
Dilutions
WB: 1:500-1:2,000, IP: 1:100-1:500
Reactivity
Human, Mouse
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 150-214 of human Cleaved PARP1 p25 (P09874).
Sequence
QLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVD
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC0091
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
27 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-Cleaved PARP1 Antibody [ARC0091] (A308342) - Antibodies.comEnlarge Image

Western Blot - Anti-Cleaved PARP1 Antibody [ARC0091] (A308342)

Western blot analysis of extracts of various cell lines, using Anti-Cleaved PARP1 Antibody [ARC0091] (A308342) at 1:1,000 dilution. Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western Blot - Anti-Cleaved PARP1 Antibody [ARC0091] (A308342) - Antibodies.comEnlarge Image

Western Blot - Anti-Cleaved PARP1 Antibody [ARC0091] (A308342)

Immunoprecipitation analysis of 600µg extracts of Jurkat cells using 3µg of Anti-Cleaved PARP1 Antibody [ARC0091] (A308342). This Western blot was performed on the immunoprecipitate using Anti-Cleaved PARP1 Antibody [ARC0091] (A308342) at a dilution of 1:500.
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