Recombinant human monoclonal antibody to Glypican 3.
Applications
ELISA, FACS, Functional Assay, In Vivo
Reactivity
Human
Host
Human
Clonality
Monoclonal
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A affinity chromatography.
Concentration
Reconstitution dependent.
Molecular Weight
This antibody has a predicted MW of 145.5 kDa.
Purity
> 95% (by SDS-PAGE and SEC-HPLC).
Product Form
Lyophilized
Reconstitution
Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulation
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
Glypican-3, GPC3, GTR2-2, Intestinal protein OCI-5, MXR7, OCI5
SDS-PAGE analysis of Codrituzumab Biosimilar - Anti-Glypican 3 Antibody - Low endotoxin, Azide free (A323365) under reducing (R) conditions confirms that the purity of this antibody is greater than 95%.
SEC-HPLC analysis of Codrituzumab Biosimilar - Anti-Glypican 3 Antibody - Low endotoxin, Azide free (A323365) confirms that the purity of this antibody is greater than 95%.
Human GPC3 CHOS cells were stained with Codrituzumab Biosimilar - Anti-Glypican 3 Antibody - Low endotoxin, Azide free (A323365) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50 = 2.142 nM.
HEP3B cells were stained with Codrituzumab Biosimilar - Anti-Glypican 3 Antibody - Low endotoxin, Azide free (A323365) and negative control protein respectively, washed and then followed by PE and analyzed with FACS.
HEPG2 cells were stained with Codrituzumab Biosimilar - Anti-Glypican 3 Antibody - Low endotoxin, Azide free (A323365) and negative control protein respectively, washed and then followed by PE and analyzed with FACS.
Codrituzumab Biosimilar - Anti-Glypican 3 Antibody - Low endotoxin, Azide free (A323365) induced ADCC activity was evaluated using human GPC3 CHOS Cell. The max Lysis rate was approximately 30%.
The endocytosis ratio of Codrituzumab Biosimilar - Anti-Glypican 3 Antibody - Low endotoxin, Azide free (A323365) by HuH-7 increased with the increase of antibody concentration, and the Internalization Rate (%) reached 40%at antibody concentration of 1 nM.
Huh-7 cells were stained with Codrituzumab Biosimilar - Anti-Glypican 3 Antibody - Low endotoxin, Azide free (A323365) and negative control protein respectively, washed and then followed by PE and analyzed with FACS.
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