Rabbit polyclonal antibody to cofilin1 (phospho-Tyr139)
Specificity
The antibody detects endogenous level of cofilin1 only when phosphorylated at tyrosine 139.
Applications
IHC
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of tyrosine 139 (N-C-Y(p)-E-E) derived from Human cofilin.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using cofilin1 (Phospho-Tyr139) Antibody #11506 (left) or the same antibody preincubated with blocking peptide (right).
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