This antibody recognises both Collagen I and III. Less than 0.5% reactivity was observed with bovine albumin and immunoglobulins.Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues.
Applications
ELISA, IF
Dilutions
ELISA: 1:2,000 - 1:8,000, IF: 1:10 - 1:40
Reactivity
Bovine
Immunogen
Bovine collagen type I and III from skin.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 500 µl of sterile distilled water or saline.
Formulation
Supplied in 0.1M Sodium Chloride with 0.01M Sodium Phosphate, 0.01M Sodium Borate, 1% Dextran, and 1% Mannitol.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rabbit anti Bovine collagen I/III antibody reacts with both Collagen I and III. Less than 0.5% reactivity was observed with bovine albumin and immunoglobulins.Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues.
