Rabbit polyclonal antibody to Connexin 43 (phospho-Ser368)
Specificity
The antibody detects endogenous level of Connexin43 only when phosphorylated at serine 368.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 368(R-A-S(p)-S-R) derived from Human Connexin 43.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Predicted MW
43kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
Connexin-43, Cx43, Gap junction 43 kDa heart protein, Gap junction alpha-1 protein, GJA1, GJAL
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Connexin 43 (Phospho-Ser367) Antibody #11258 (left) or the same antibody preincubated with blocking peptide (right).
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