Immunohistochemistry analysis of a formalin fixed paraffin embedded human brain cortex section with Anti-Coronin 1a Antibody (A85431) at a dilution of 1:2,000 detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Counterstained with Hematoxylin (blue). The Anti-Coronin 1a Antibody (A85431) specifically labels the perikarya and processes of microglial cells. Note: this antibody performs well in testing with 4% PFA and standard NBF fixed mouse, rat, and human tissue.
Immunocytochemistry of a mixed neuron/glial culture from newborn rat brain stained with Anti-Coronin 1a Antibody, at a dilution of 1:10,000, in green, and Anti-Vimentin Antibody (A85421 | 1:10,000, in red. Blue is nuclear DNA counter stain. Glial cells and fibroblasts stain with vimentin, while microglia alone stain strongly and specifically for Coronin 1a, which can therefore be used as a robust marker of this important cell type.
Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with Anti-Coronin 1a Antibody (A85431), at a dilution of 1:1,000 in red, and co-stained with Anti-GFAP Antibody (A85422), at a dilution of 1:1,000 in green. The nuclear DNA is visualised in blue using Hoechst staining. The Anti-Coronin 1a Antibody (A85431) labels protein expressed in the cytoplasm of microglia cells, while Anti-GFAP Antibody (A85422) stains intermediate filaments in astrocytic cells.
Western blot analysis of tissue lysates using Anti-Coronin 1a Antibody (A85431), at a dilution of 1:5,000, in green. The lanes contain samples of: [Lane 1] Protein standards, [Lane 2] mouse brain, [Lane 3] rat brain, [Lane 4] cow cerebellum, [Lane 5] cow cortex, and [Lane 6] pig spinal cord. The strong single band at approximately 55 kDa corresponds to the coronin 1a protein.
Western blot of HL60 cell extract stained with Anti-Coronin 1a Antibody, at a dilution of 1:10,000). A prominent band running with an apparent SDS-PAGE molecular weight of ~57 kDa corresponds to Coronin 1a. Numbers represent positions of molecular weight standards in kDa.
