Anti-COX1 / Cyclooxygenase 1 Antibody (A305018)

Name: Anti-COX1 / Cyclooxygenase 1 Antibody
Brand: Antibodies.com
SKU: A305018

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This product is discontinued

Anti-COX1 / Cyclooxygenase 1 Antibody (A305018) has been discontinued and is no longer available.

View all Primary Antibodies.

Specifications

Name

Anti-COX1 / Cyclooxygenase 1 Antibody

Description

Rabbit polyclonal antibody to COX1 / Cyclooxygenase 1.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide of Human Cyclooxygenase.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Molecular Weight

~75 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

COX 1, COX 3, COX-1, COX1, Cox3, Cyclooxygenase 1, Cyclooxygenase 3, included, Cyclooxygenase-1, EC 1.14.99.1, Partial COX1 proteins, included, PCOX1, PGG/HS, PGH synthase 1, PGH1_HUMAN, PGHS-1, PGHS1, PHS 1, PHS1, Prostaglandin G/H synthase 1, Prostaglandin H2 synthase 1, Prostaglandin-endoperoxide synthase 1, Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase), PTGHS, PTGS1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Immunofluorescence - Anti-COX1 / Cyclooxygenase 1 Antibody (A305018)

Immunocytochemistry/Immunofluorescence analysis of human colon carcinoma cell line (RKO), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-COX1 / Cyclooxygenase 1 Antibody (A305018), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Microsome Membrane, Endoplasmic Reticulum Membrane, Membrane. Magnification: 60X.(A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cyclooxygenase 1 Antibody. (D) Composite.

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Western Blot - Anti-COX1 / Cyclooxygenase 1 Antibody (A305018)

Western blot analysis of rat brain cell lysates showing detection of 68.7 kDa Cyclooxygenase 1 protein using Anti-COX1 / Cyclooxygenase 1 Antibody (A305018) at 1:1,000 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat brain cell lysates. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: 68.7 kDa.

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Western Blot - Anti-COX1 / Cyclooxygenase 1 Antibody (A305018)

Western blot analysis of mouse kidney cell lysates showing detection of 68.7 kDa Cyclooxygenase 1 protein using Anti-COX1 / Cyclooxygenase 1 Antibody (A305018) at 1:1,000 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder (MW). Lane 2: mouse kidney cell lysates. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: 68.7 kDa.

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Western Blot - Anti-COX1 / Cyclooxygenase 1 Antibody (A305018)

Western blot analysis of human HeLa and HEK293Trap cell lysates showing detection of 68.7 kDa Cyclooxygenase 1 protein using Anti-COX1 / Cyclooxygenase 1 Antibody (A305018) at 1:1,000 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder (MW). Lane 2: human HeLa and 293Trap cell lysates. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: 68.7 kDa.