Anti-COX2/Cyclooxygenase 2 Antibody (A83806)

COX2/Cyclooxygenase 2 expression in A549 (A) and Daudi (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-COX2/Cyclooxygenase 2 Antibody (A83806) at 0.1µg/ml and detected by chemiluminescence.

COX2/Cyclooxygenase 2 expression in HepG2 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-COX2/Cyclooxygenase 2 Antibody (A83806) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic/vesicle staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

COX2/Cyclooxygenase 2 expression in NIH3T3 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-COX2/Cyclooxygenase 2 Antibody (A83806) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and vesicle staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

COX2/Cyclooxygenase 2 expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-COX2/Cyclooxygenase 2 Antibody (A83806) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.