Western Blot - Anti-Creatine Kinase MM Antibody (A85110)
Creatine Kinase MM expression in Human (A, C) and Mouse (B, D) Skeletal Muscle (A, B) and Heart (C, D) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Creatine Kinase MM Antibody (A85110) at 0.01µg/ml and detected by chemiluminescence.
Immunohistochemistry - Anti-Creatine Kinase MM Antibody (A85110)
Creatine Kinase MM expression in Human Heart analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Creatine Kinase MM Antibody (A85110) at 5µg/ml and revealed with alkaline phosphatase (AP).
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