This antibody targets the C-terminal region of human CRHR1, recognizing the non-phosphorylated isoform. It is suitable for Western blot analysis, enables immunoprecipitation from cell and tissue lysates, and is optimized for immunohistochemistry in cultured cells and tissue sections.
Applications
WB, ICC, IHC
Dilutions
WB: 1,000, IHC: 1:100, ICC: 1:200
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C-terminal of human CRHR1. Immunogen range is 22-22 amino acids.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunohistochemical localization of Corticotropin-Releasing Factor Receptor 1 (CRF1) in anterior pituitary. Pituitary sections were dewaxed, microwaved in citric acid, and stained with anti-CRF1 antibody (A334474) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed with 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. CRF1 receptors were detected at the plasma membrane of a subset of anterior pituitary cells.
Western blot confirmation of Corticotropin-Releasing Factor Receptor 1 (CRF1) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing CRF1 were probed with the phosphorylation-independent anti-CRF1 antibody (A334474) at a 1:1000 dilution.
Immunocytochemical analysis of Corticotropin-Releasing Factor Receptor 1 (CRF1) in HEK293 cells. HEK293 cells stably expressing CRF1 were untreated or treated with 100 nM CRF for 30 min and stained with anti-CRF1 antibody (A334474) at 1:200. CRF1 receptors were localized to the plasma membrane in untreated cells (0 min) and in perinuclear vesicular clusters after 30 min of CRF exposure.
Publishing research using Anti-CRHR1 Antibody (A334474)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-CRHR1 Antibody (A334474)
