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Anti-CRISPR-Cas12j Antibody [5F95] (A270595)

Immunofluorescence - Anti-CRISPR-Cas12j Antibody [5F95] (A270595) - Antibodies.com Western Blot - Anti-CRISPR-Cas12j Antibody [5F95] (A270595) - Antibodies.com
$190
Product Datasheet

Mouse monoclonal [5F95] antibody to CRISPR-Cas12j for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-CRISPR-Cas12j Antibody [5F95]
Description
Mouse monoclonal [5F95] antibody to CRISPR-Cas12j.
Applications
WB, ICC/IF
Dilutions
WB: 1:1,000-1:2,000, ICC/IF: 1:1,000
Immunogen
Recombinant full-length Cas-12j, expressed in and purified from E. coli.
Sequence
MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEFMPKPAVESEFSKVLKKHFPGERFRSSYMKRGGKILAAQGEEAVVAYLQGKSEEEPPNFQPPAKCHVVTKSRDFAEWPIMKASEAIQRYIYALSTTERAACKPGKSSESHAAWFAATGVSNHGYSHVQGLNLIFDHTLGRYDGVLKKVQLRNEKARARLESINASRADEGLPEIKAEEEEVATNETGHLLQPPGINPSFYVYQTISPQAYRPRDEIVLPPEYAGYVRDPNAPIPLGVVRNRCDIQKGCPGYIPEWQREAGTAISPKTGKAVTVPGLSPKKNKRMRRYWRSEKEKAQDALLVTVRIGTDWVVIDVRGLLRNARWRTIAPKDISLNALLDLFTGDPVIDVRRNIVTFTYTLDACGTYARKWTLKGKQTKATLDKLTATQTVALVAIDLGQTNPISAGISRVTQENGALQCEPLDRFTLPDDLLKDISAYRIAWDRNEEELRARSVEALPEAQQAEVRALDGVSKETARTQLCADFGLDPKRLPWDKMSSNTTFISEALLSNSVSRDQVFFTPAPKKGAKKKAPVEVMRKDRTWARAYKPRLSVEAQKLKNEALWALKRTSPEYLKLSRRKEELCRRSINYVIEKTRRRTQCQIVIPVIEDLNVRFFHGSGKRLPGWDNFFTAKKENRWFIQGLHKAFSDLRTHRSFYVFEVRPERTSITCPKCGHCEVGNRDGEAFQCLSCGKTCNADLDVATHNLTQVALTGKTMPKREEPRDAQGTAPARKTKKASKSKAPPAEREDQTPAQEPSQTS
Host
Mouse
Clonality
Monoclonal
Clone ID
5F95
Isotype
IgG1
Conjugate

Unconjugated

Purification
Immunogen affinity purification.
Concentration
1 mg/ml
Molecular Weight
90 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Immunofluorescence - Anti-CRISPR-Cas12j Antibody [5F95] (A270595) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-CRISPR-Cas12j Antibody [5F95] (A270595)

Immunofluorescent analysis of HEK293 cells transfected with pCI-Neo-Mod vector including DNA encoding the Cas-Phi protein, stained with Anti-CRISPR-Cas12j Antibody [5F95] (A270595), at a dilution of 1:2,000, in red, and co-stained with Anti-Vimentin Antibody (A85420), at a dilution of 1:2,000, in green. The blue is Hoechst staining of nuclear DNA. Anti-CRISPR-Cas12j Antibody [5F95] (A270595) reveals expression of the Cas-phi protein only in transfected cells, while Anti-Vimentin Antibody (A85420) stains the cytoskeletal intermediate filament network in all cells.
Western Blot - Anti-CRISPR-Cas12j Antibody [5F95] (A270595) - Antibodies.comEnlarge Image

Western Blot - Anti-CRISPR-Cas12j Antibody [5F95] (A270595)

Western blot analysis of HEK293 cell lysates using Anti-CRISPR-Cas12j Antibody [5F95] (A270595), at a dilution of 1:1,000, in green. The lanes contain: [Lane 1] protein standard, [Lane 2] non-transfected cells, [Lane 3] cells transfected with pCI-Neo-Mod vector containing full length Cas-phi cDNA, and [Lane 4] cells transfected with pCI-Neo-GFP vector containing full length Cas-phi cDNA. The band at about 90 kDa demonstrates expression of Cas-phi protein and the band at about 120 kDa corresponds to GFP-Cas-phi fusion protein.
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