Western Blot - Anti-CrkL (phospho Tyr207) Antibody (A89607)
Western blot analysis of extracts of K-562 cells, using Anti-CrkL (phospho Tyr207) Antibody (A89607) at 1:1,000 dilution or CRKL antibody (A11735). K-562 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 5s.
