Western Blot - Anti-CSF-1-R (phospho Tyr708) Antibody (A11085)
Western blot analysis of extracts of K-562 cells, using Anti-CSF-1-R (phospho Tyr708) Antibody (A11085) at 1:1,000 dilution. K-562 cells were treated by G-CSF (25 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
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