This antibody reacts with Csk (C-terminal Src kinase), a 50 kDa non-receptor protein tyrosine kinase expressed in cytosol.
Applications
WB, IP, ICC
Dilutions
IHC: Positive Control: HeLa human cervix carcinoma cell line, WB: The recommended dilution of the antibody CSK-04 is 1:250-1:300; weak non-specific band of cca 60 kDa is detected in human cell lines in lower antibody dilutions, IP: The immunosorbent (CSK-04 antibody covalently bound to beads) immunoprecipitates Csk from cell lysates.
Reactivity
Human, Mouse
Immunogen
Bacterially expressed recombinant fragment of human Csk corresponding to aa 330-450.
Host
Mouse
Clonality
Monoclonal
Clone ID
CSK-04
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Predicted MW
50 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunocytochemistry staining of Csk in human HeLa cell line using Anti-CSK Antibody [CSK-04] (A86870) green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Publishing research using Anti-CSK Antibody [CSK-04] (A86870)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-CSK Antibody [CSK-04] (A86870)