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Anti-Cullin 4A/CUL-4A Antibody (A14234)

Western Blot - Anti-Cullin 4A/CUL-4A Antibody (A14234) - Antibodies.com Western Blot - Anti-Cullin 4A/CUL-4A Antibody (A14234) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to Cullin 4A/CUL-4A for WB, ICC/IF and IP.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Cullin 4A/CUL-4A Antibody
Description
Rabbit polyclonal antibody to Cullin 4A/CUL-4A.
Applications
WB, ICC/IF, IP
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:100, IP: 1:500-1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Cullin 4A (Q13619).
Sequence
IERERSGEAVDRSLLRSLLGMLSDLQVYKDSFELKFLEETNCLYAAEGQRLMQEREVPEYLNHVSKRLEEEGDRVITYLDHSTQKPLIACVEKQLLGEHLT
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
80 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-Cullin 4A/CUL-4A Antibody (A14234) - Antibodies.comEnlarge Image

Western Blot - Anti-Cullin 4A/CUL-4A Antibody (A14234)

Western blot analysis of extracts of various cell lines, using Anti-Cullin 4A/CUL-4A Antibody (A14234) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Western Blot - Anti-Cullin 4A/CUL-4A Antibody (A14234) - Antibodies.comEnlarge Image

Western Blot - Anti-Cullin 4A/CUL-4A Antibody (A14234)

Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg of Anti-Cullin 4A/CUL-4A Antibody (A14234). This Western blot was performed on the immunoprecipitate using Anti-Cullin 4A/CUL-4A Antibody (A14234) at a dilution of 1:1000.
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