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Anti-Cullin 4A/CUL-4A Antibody [ARC1237] (A305881)

Western Blot - Anti-Cullin 4A/CUL-4A Antibody [ARC1237] (A305881) - Antibodies.com Immunohistochemistry - Anti-Cullin 4A/CUL-4A Antibody [ARC1237] (A305881) - Antibodies.com
$485
Product Datasheet

Rabbit monoclonal [ARC1237] antibody to Cullin 4A/CUL-4A for WB and IHC.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Cullin 4A/CUL-4A Antibody [ARC1237]
Description
Rabbit monoclonal [ARC1237] antibody to Cullin 4A/CUL-4A.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reactivity
Human
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 660-759 of human Cullin 4A (Q13619).
Sequence
FKHKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAIVRIMKMRKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLIDRDYMERDKDNPNQYHYVA
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1237
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
88 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-Cullin 4A/CUL-4A Antibody [ARC1237] (A305881) - Antibodies.comEnlarge Image

Western Blot - Anti-Cullin 4A/CUL-4A Antibody [ARC1237] (A305881)

Western blot analysis of extracts of various cell lines, using Anti-Cullin 4A/CUL-4A Antibody [ARC1237] (A305881) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry - Anti-Cullin 4A/CUL-4A Antibody [ARC1237] (A305881) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Cullin 4A/CUL-4A Antibody [ARC1237] (A305881)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Cullin 4A/CUL-4A Antibody [ARC1237] (A305881) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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