This antibody is predicted to recognize both reported isoforms (NP_001164645.1 and NP_001328.1). The listed variants correspond to the same protein sequence, represented by NP_001164643.1, NP_001328.1 and NP_001164642.1.
Applications
ELISA, WB
Dilutions
ELISA: 1:128,000, WB: 1-3µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the internal region (near N terminus), amino acids 34-47, of human CX3CR1.
Sequence
C-DQFPESVTENFEYD
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
39 kDa
Predicted MW
40.4kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
CX3CR1 expression in Kelly (A) and U251-MG (B) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-CX3CR1 Antibody (A82937) at 2µg/ml and detected by chemiluminescence.
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