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Anti-Cyan Fluorescent Protein Antibody (A283797)

This product is discontinued

Anti-Cyan Fluorescent Protein Antibody (A283797) has been discontinued and is no longer available.

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Name
Anti-Cyan Fluorescent Protein Antibody
Description
Rabbit polyclonal antibody to Cyan Fluorescent Protein.
Specificity
This antibody recognises the cyan fluorescent protein (CFP), a monomeric engineered derivate of green fluorescent protein (GFP) isolated from the jellyfish Aequorea victoria. CFP is a ~31 kDa protein that is optimally excited at a wavelength of 439 nm and emits light at 476 nm. In FRET (Fluorescence/Förster Resonance Energy Transfer) experiments, CFP is often used in combination with YFP (yellow fluorescent protein).
Applications
WB
Dilutions
WB: 0.5 - 4.0 µg/ml
Immunogen
E. coli derived recombinant cyan fluorescent protein.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Concentration
500 µg/ml
Molecular Weight
Approximately 31 kDa on recombinant CFP. The observed band size is approximately 35 kDa. This antibody has minimum cross-reactivity with Yellow Fluorescent Protein.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 30% Glycerol, 0.5% BSA, and 0.01% Thiomersal.
Storage
Store undiluted at -20°C. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rabbit anti CFP antibody recognizes the cyan fluorescent protein (CFP), a monomeric engineered derivate of green fluorescent protein (GFP) isolated from the jellyfish Aequorea victoria. CFP is a ~31 kDa protein that is optimally excited at a wavelength of 439 nm and emits light at 476 nm (Day and Davidson 2009). In FRET (Fluorescence/Förster Resonance Energy Transfer) experiments, CFP is often used in combination with YFP (yellow fluorescent protein) (Karpova and McNally 2006).
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.