Mouse monoclonal [V152] antibody to Cyclin B1 (PE).
Specificity
This antibody recognizes cyclin B1, a 48 kDa intracellular protein necessary for G2/M phase transition of the cell cycle.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests; Intracellular staining.
Reactivity
Mammalian
Immunogen
Recombinant His-tagged hamster cyclin B1.
Host
Mouse
Clonality
Monoclonal
Clone ID
V152
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
48 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of K562 cells stained with Anti-Cyclin B1 Antibody [V152] (PE) (10 µl reagent per million cells in 100 µl of cell suspension, red-filled) from K562 cells stained with Mouse IgG1 [MOPC-21] (APC) - Isotype Control (concentration in sample is 6 µg/ml, same as Anti-Cyclin B1 Antibody [V152] (PE) concentration, black-dashed) in flow cytometry analysis (intracellular staining) of K562 cell suspension.
Publishing research using Anti-Cyclin B1 Antibody [V152] (PE) (A285829)? Please let us know so that we can list the citation on this page.
Proteins predicted to interact with Cyclin B1
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
![Flow Cytometry - Anti-Cyclin B1 Antibody [V152] (PE) (A285828) - Antibodies.com](https://cdn.antibodies.com/image/catalog/285/A285829_1.jpg?profile=product_top)
![Flow Cytometry - Anti-Cyclin B1 Antibody [V152] (PE) (A285828) - Antibodies.com](https://cdn.antibodies.com/image/catalog/285/A285829_1.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-Cyclin B1 Antibody [V152] (PE) (A285828) - Antibodies.com](https://cdn.antibodies.com/image/catalog/285/A285829_1.jpg?profile=product_image)