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Anti-Cyclin B2/CCNB2 Antibody [ARC1435] (A306931)

Western Blot - Anti-Cyclin B2/CCNB2 Antibody [ARC1435] (A306931) - Antibodies.com Immunohistochemistry - Anti-Cyclin B2/CCNB2 Antibody [ARC1435] (A306931) - Antibodies.com Immunofluorescence - Anti-Cyclin B2/CCNB2 Antibody [ARC1435] (A306931) - Antibodies.com
$485
Product Datasheet

Rabbit monoclonal [ARC1435] antibody to Cyclin B2/CCNB2 for WB, IHC and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Cyclin B2/CCNB2 Antibody [ARC1435]
Description
Rabbit monoclonal [ARC1435] antibody to Cyclin B2/CCNB2.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cyclin B2 (O95067).
Sequence
MALLRRPTVSSDLENIDTGVNSKVKSHVTIRRTVLEEIGNRVTTRAAQVAKKAQNTKVPVQPTKTTNVNKQLKPTASVKPVQMEKLAPKGPSPTPEDVSM
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1435
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
45 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-Cyclin B2/CCNB2 Antibody [ARC1435] (A306931) - Antibodies.comEnlarge Image

Western Blot - Anti-Cyclin B2/CCNB2 Antibody [ARC1435] (A306931)

Western blot analysis of extracts of Rat testis cells, using Anti-Cyclin B2/CCNB2 Antibody [ARC1435] (A306931) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Anti-Cyclin B2/CCNB2 Antibody [ARC1435] (A306931) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Cyclin B2/CCNB2 Antibody [ARC1435] (A306931)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Cyclin B2/CCNB2 Antibody [ARC1435] (A306931) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-Cyclin B2/CCNB2 Antibody [ARC1435] (A306931) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-Cyclin B2/CCNB2 Antibody [ARC1435] (A306931)

Immunofluorescence analysis of NIH-3T3 cells using Anti-Cyclin B2/CCNB2 Antibody [ARC1435] (A306931) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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